NGS微缩化应用dynamic duo组合方案.
Illumina, PacBio, Nanopore…助力提升NGS建库流程通量
使用mosquito®和dragonfly®自动化液体处理工作站，通过实现反应体系微缩化，为您突破通量及成本方面的瓶颈，让基因组学研究人员能够处理更多样品并节省试剂用量。
我们的“灵活组合”结合了mosquito的高精度固相置换式移液技术，以及dragonfly的非接触式纳升至毫升级分液技术。mosquito能够完成移液、分液、混匀、梯度稀释等操作，dragonfly则提供精准的纳升级分液，无视液体粘度及环境条件。针对越来越多的NGS建库方法及 qPCR/PCR 体系构建流程，SPTLabtech提供了稳定可靠的微缩化解决方案，提高您的工作流程效率。
mosquito® Gen3
mosquito系列中设计紧凑且易于使用的一款液体处理工作站，能够无视液体类型完成500nL至5μL的高精度多通道液体转移 通过快速的板对板液体转移和枪头更换最大化速度和效率，让基因组学研究人员在多种应用范围内受益于微缩化应用，并显著提升工作效率。
mosquito® HV
mosquito HV genomics能够完成500 nL至5µL的高精度多通道移液，适用于各类粘稠及挥发性液体的转移。加设两个板位的设计，能够满足更大通量及更高移液能力的需求，自动化高效运行完整的微缩化工作流程。
dragonfly® discovery
dragonfly® discovery采用创新的固相置换技术，能够完成200nL-4mL的高精度分液，在基因组学、分析方法开发和3D细胞培养等多种领域中实现超微量的试剂分配。30秒内完成384孔板分液，超低死体积，能够显著降低试剂及时间成本，并通过标准化工作流程以获得更高质量的数据结果。
工作流程案例
40+种微缩化NGS建库方法
| 应用领域 | 试剂名称 | 仪器型号 | 标准体积[µL] | 微缩化体积[µL] | 微缩倍数 | 引文 |
| SINGLE CELL/LOW INPUT CDNA SYNTHESIS (full transcriptome) | SMART-Seq2 | mosquito Gen3 mosquito HV genomics mosquito LV genomics dragonfly discovery | 25 | 2.5 | Up to 10x | |
| SMART-Seq V3 | mosquito Gen3 mosquito HV genomics | 7 | 3.5 | Up to 2x | ||
| Takara SMART-Seq v4 | mosquito Gen3 mosquito HV genomics | 50 | 12.5 | 4x | Paper | |
| Takara SMART-Seq Single Cell | mosquito Gen3 mosquito HV genomics | 50 | 7 | ~7x | Paper | |
| Takara SMART-Seq Stranded | mosquito Gen3 mosquito HV genomics | 100 | 25 | 4x | Webinar | |
| SINGLE CELL/LOW INPUT CDNA SYNTHESIS(3’ counting) | CEL-seq2 | mosquito LV genomics | 12 | 2.6 | ~5x | |
| Ribo-seq | mosquito LV genomics | 5.79 | 5.79 | – | Paper Paper | |
| SORT-seq | mosquito LV genomics | 26 | 2.6 | – | Paper | |
| SINGLE CELL/LOW INPUT WHOLE GENOME AMPLIFICATION | Qiagen REPLI-g Single Cell | mosquito LV genomics | 50 | 1 | 50x | Paper |
| DigiPico | mosquito LV genomics | 6.2 | 6.2 | – | Paper | |
| DNA SEQUENCING | Illumina Nextera XT | mosquito Gen3 mosquito HV genomics mosquito LV genomics | 50 | 2 | Up to 25x | Paper Paper |
| Illumina DNA Prep | mosquito Gen3 mosquito HV genomics dragonfly discovery | 50 | 5 | Up to 10x | Paper | |
| Invitrogen Collibri ES DNA | mosquito Gen3 mosquito HV genomics | 50 | 5 | 10x | Presentation | |
| NEBNext Ultra II FS DNA | mosquito Gen3 mosquito HV genomics | 50 | 5 | 10x | Webinar | |
| KAPA HyperPlus | mosquito Gen3 mosquito HV genomics | 100 | 10 | 10x | Paper | |
| modified Twist Library Preparation EF Kit | mosquito Gen3 mosquito HV genomics | 48.75 | 4.9 | Up to 10x | ||
| RNA SEQUENCING | NEBNext Ultra II Directional RNA | mosquito Gen3 mosquito HV genomics | 50 | 5 | 10x | Paper |
| NEBNext Multiplex Small RNA | mosquito LV genomics | 100 | 20 | 5x | Paper Paper | |
Takara SMARTer Stranded Total RNA-Seq | mosquito Gen3 mosquito HV genomics | 100 | 12.5 | 8x | Paper | |
| AMPLICON SEQUENCING | COVID-seq | mosquito Gen3 mosquito HV genomics dragonfly discovery | 50 | 25 | 2x | Paper Webinar |
| Illumina 16S Library Prep | mosquito Gen3 mosquito HV genomics | 50 | 5 | 10x | Webinar | |
Ion AmpliSeq Library Kit 2.0 | mosquito Gen3 mosquito HV genomics mosquito LV genimocs | 30 | 2.5 | Up to 12.5x | Paper | |
| SAMPLE AND LIBRARY BEAD CLEAN-UP, QUANTIFICATION | Beckman AMPure XP, SPRIselect | mosquito Gen3 mosquito HV genomics dragonfly discovery | variable | >10x | Up to 10x | Paper Paper |
| Biotium AccuClear | mosquito Gen3 mosquito HV genomics mosquito LV genimocs | variable | variable | Up to 15x | Paper | |
Invitrogen Collibri Library Quant | mosquito Gen3 mosquito HV genomics | 20 | 2.5 | Up to 8x | ||
Promega QuantiFluor | mosquito Gen3 mosquito HV genomics mosquito LV genimocs | variable | variable | Up to 30x | Paper | |
Quant-iT PicoGreen | mosquito Gen3 mosquito HV genomics mosquito LV genimocs | variable | variable | Up to 150x | App note | |
Roche KAPA Library Quant | mosquito Gen3 mosquito HV genomics | 20 | 2.5 | Up to 8x | ||
| qPCR/PCR SETUP | gene expression profiling (Fluidigm Biomark HD) | mosquito Gen3 mosquito HV genomics | 23 | – | – | Paper |
| other | mosquito Gen3 mosquito HV genomics mosquito LV genimocs | variable | variable | Up to 50x | Paper Technical note | |
| OTHER | Olink Explore | mosquito LV genomics dragonfly discovery | 19.8 | – | – | Paper |
| HiFiViral SARS-CoV-2 | mosquito LV genomics dragonfly discovery | – | – | – | Paper |